Time-lapse imaging of individual FUCCI CD+8 T cells

Time-lapse imaging of individual Fucci CD8+ T cells. Cell Trace Violet-labeled Fucci-CD8+ T cells were stimulated with plate-bound anti-CD3ε (1.0 μg/ml) and anti-CD28 mAb (0.5 μg/ml) and sorted to collect the cells in the 8th division generation. Sorted cells were seeded into microgrid arrays in the presence of rIL-2 (10 ng/ml) and subjected to time-lapse imaging every 3-4 min with 6-8 stacks at 0.8-1.2 μm (z-plane). Representative movie from 1st day of imaging showing one area from multiple positioning. Movie duration: 16 h.

The videos are from the paper "Real time tracking of cell progression during CD8+ effector and and memory T-cell differentiation" published in Nature Communications.

http://www.nature.com/ncomms/2015/150224/ncomms7301/full/ncomms7301.html

Sequential division of smaller or larger sized T cells

Sequential divisions of smaller or larger sized T cells. Cell Trace Violet-labeled Fucci-CD8+ T cells were stimulated with plate-bound anti-CD3ε (1.0 μg/ml) and anti-CD28 mAb (0.5 μg/ml) and sorted to collect the cells in the 8th division generation. Sorted cells were seeded into microgrid arrays in the presence of rIL-2 (10 ng/ml) and subjected to time-lapse imaging every 3-4 min with 6-8 stacks at 0.8-1.2 μm (z-plane). Representative movie with smaller and larger size cells from 3rd day of imaging of sorted 8th generation. Movie duration: 3.5 hr.

The videos are from the paper "Real time tracking of cell progression during CD8+ effector and and memory T-cell differentiation" published in Nature Communications.

http://www.nature.com/ncomms/2015/150224/ncomms7301/full/ncomms7301.html

Imaging thymocyte and stromal cell-cell interactions

Composite fluorescence and DIC time lapse movie of a thymocyte interacting with stromal cells. The extract from the movie allows the cell-cell interactions which were captured over 22 hours.

The videos are from the paper "A Method for Prolonged Imaging of Motile Lymphocytes" published in the journal of Immunology and Cell Biology.

http://www.nature.com/icb/journal/v87/n2/abs/icb200879a.html

Imaging T cell and APC cell-cell interactions

Time lapse DIC movie of a T cell interacting with an antigen presenting cell (APC). The extract from the movie shows the ability to capture the cell-cell interactions on a single cell level over 22 hours.

The videos are from the paper "A Method for Prolonged Imaging of Motile Lymphocytes" published in the journal of Immunology and Cell Biology.

http://www.nature.com/icb/journal/v87/n2/abs/icb200879a.html

Time lapse microscopy of live cells with microgrid arrays

Time lapse DIC movie of MLA-144 cells using a 250um microgrid array. The extract of the movie shows the isolation of sub-populations of cells for single cell analysis over 22 hours.

The videos are from the paper "A Method for Prolonged Imaging of Motile Lymphocytes" published in the journal of Immunology and Cell Biology.

http://www.nature.com/icb/journal/v87/n2/abs/icb200879a.html

Traditional ime lapse microscopy of live cells without microgrid arrays

Time lapse DIC movie of MLA-144 cells without using microgrid arrays. The extract from the movie shows the movement and aggregation of the cells over 22 hours.

The videos are from the paper "A Method for Prolonged Imaging of Motile Lymphocytes" published in the journal of Immunology and Cell Biology.

http://www.nature.com/icb/journal/v87/n2/abs/icb200879a.html